Journal: Kidney Medicine

Article Title: A Large Deletion With a Large Impact: Homozygous 5,600 bp Deletion of the GALNT3 Gene Causing Hyperphosphatemic Tumoral Calcinosis

doi: 10.1016/j.xkme.2026.101241

Figure Lengend Snippet: Impact of GalNAc-T3 enzyme dysfunction; normal situation (left), deletion leading to GalNAc-T3 dysfunction (right). FGF-23, fibroblast growth factor 23; FGFR, fibroblast growth factor receptor.

Article Snippet: Intact FGF23 was measured with the human intact FGF23 enzyme-linked immunosorbent assay (#60-6600, Lot 173373, Quidel Corporation) according to the manufacturer’s protocol.

Techniques:

Journal: JBMR Plus

Article Title: Multi-tissue spatial transcriptomics identified simultaneous responses to oxidative stress and apoptosis in parallel with tissue-specific reprogramming in modeled chronic kidney disease-mineral and bone disorder

doi: 10.1093/jbmrpl/ziaf161

Figure Lengend Snippet: Spatial transcriptomics identified tissue subtypes in muscle and bone. (A-C) Plasma intact FGF23 and serum TNF and blood urea nitrogen (BUN) levels in control (Ctrl) and CKD-MBD (CKD) mice (* p < .05; ** p < .01; *** p < .001 vs controls). H&E bone-muscle cross-sections for Ctrl (D) and CKD (E) and the Seurat clusters mapped on the sections (F and G). (H) Tissue and fiber type specific marker gene expression. (I) UMAP of Ctrl and CKD samples, with major tissue and muscle fiber types labeled. (J) Localization of major muscle fiber types in Ctrl and CKD tissue sections (colors correspond to UMAP in H).

Article Snippet: Plasma FGF23 was assessed using a rodent specific commercial ELISA for bioactive, intact FGF23 (“iFGF23”; Quidel, Inc.).

Techniques: Clinical Proteomics, Control, Marker, Gene Expression, Labeling

Journal: Cell Biochemistry and Biophysics

Article Title: Chorein Regulates Key Osteoblast Genes in UMR-106 Cells

doi: 10.1007/s12013-025-01939-4

Figure Lengend Snippet: Effect of CRISPR/Cas9-induced Vps13a knockdown on FGF23 production. ( A ) Fgf23 mRNA ( n = 7) expression normalized to β-actin ( Actb ) in control cells transfected with non-targeting (NT) sequence and Vps13a knockdown (KD) cells using CRISPR/Cas9 system. ( B ) C-terminal FGF23 protein concentration ( n = 8) in the cell culture supernatant of NT and Vps13a knockdown cells. All data are shown as arithmetic means ± SEM. (A: Unpaired Student’s t -test; B: Mann-Whitney U test)

Article Snippet: The concentration of FGF23 protein in the cell culture supernatant was determined using ELISA kits (mouse/rat FGF23 (C-Term) and mouse/rat FGF23 (intact), both from Quidel, Cologne, Germany).

Techniques: CRISPR, Knockdown, Expressing, Control, Transfection, Sequencing, Protein Concentration, Cell Culture, MANN-WHITNEY

Journal: Cell Biochemistry and Biophysics

Article Title: Chorein Regulates Key Osteoblast Genes in UMR-106 Cells

doi: 10.1007/s12013-025-01939-4

Figure Lengend Snippet: Knockdown of Vps13a influences mRNA expression of FGF23 regulators . Relative mRNA expression of Phex ( A , n = 6) and Galnt3 ( B , n = 8) mRNA normalized to β-actin ( Actb ) in non-targeting (NT) control cells and Vps13a knockdown (KD) cells. All data are shown as arithmetic means ± SEM. (A: Unpaired t -test with Welch’s correction; B: Unpaired Student’s t -test)

Article Snippet: The concentration of FGF23 protein in the cell culture supernatant was determined using ELISA kits (mouse/rat FGF23 (C-Term) and mouse/rat FGF23 (intact), both from Quidel, Cologne, Germany).

Techniques: Knockdown, Expressing, Control